Definition
Polypeptide is a biologically active substance that is associated with various cellular functions in a living body. It consists of a variety of amino acids and is always applied in functional analysis, antibody research, especially drug development and other fields.
History
It was Merrifield who proposed synthesis of solid - phase peptides (SPPS), which is designed with convenient and fast features as a milestone in polypeptide organic synthesis.
Synthesizer types
Due to the complicated procedures and time consuming of polypeptide synthesis, automatic peptide synthesizers are developed. They can be classified into two categories: one is targeting at application of small number but large synthesis amount, the other is focusing on drug research and development, gene research and other scientific applications.
Recently, there are all kinds of synthesizers, it can be microkine, milligrams, gram and kilograms according to the amount of synthesis, it can be research type, small test type, pilot type, general production type and GMP production type according to different functions, it can be fully-automatic, semi-automatic and manual according to degree of automation, etc.
Operating principle
Theory of peptide synthesizers is just like the reaction principle of solid-phase synthesis. In a closed explosion-proof glass reactor, it makes amino acid continuous addition, reaction, synthesis, operation in accordance with the known order and finally gets the peptide carrier. The reaction principle of solid-phase synthesis reduces the difficulty of each step for product purification greatly. It also protective for side reactions and amino acid side chain.
Since the propose of solid-phase synthesis method, it has become the most popular technique for synthesizing polypeptides and protein through continuous development and improvment. In a word, polypeptide synthesis includes solid-phase synthesis and liquid-phase peptide synthesis.
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