Referring to biological experiments, the most difficult thing is to prepare and analyze all kinds of samples. This also gives a reason for a majority of biotech companies to mushroom, like Creative Proteomics. This blog is used to introduce 3 kinds of applications used in proteomics fields.
Absolute quantification (AQUA)
Absolute quantification is a technique used in protein quantification. By virtue of its advantages, absolute quantification can exhibit robust efficacy and is being widely applied in a variety of proteomics studies. Its general procedure is for the quantification of proteins and post-translational modification, and it provides an absolute quantification by utilizing synthetic peptides with stable isotopes. This service is equipped with features of simplicity, sensitivity. What’s more, it is also coupled with the widespread availability of tandem mass spectrometers. This also makes aqua proteomics technique become the most useful strategy for protein measurement and post-translational modifications.
Protein Therapeutic Analysis
Protein therapeutic analysis is mainly including recombinant proteins, monoclonal antibodies and peptides analysis. They all are successfully used in clinical and their potential has received unprecedented recognition among scientists. There are some items needed to be prepared in prior to guarantee the correct synthesis and modifications of protein therapeutics analysis. They are peptide mapping. Amino acid analysis, thiol and disulfide bonds characterization and so on.
Peptidomics
Peptidomics is defined as the systematic comprehensive, qualitative/quantitative multiplex analysis of endogenous peptides in biological samples, which are collected at a chosen time point and location. Peptidomics can act as a bridge between proteomics and metabolomics. What’s more, it can complement proteomics. With the help of peptidomics studies, a wealth of information about disease states, toxicity and drug efficacy. During the peptidomics research, the sample preparation usually needs to remove most of hyper-abundant proteins before analysis. And it also has the ability in biomarker discovery.
These services belonging to proteomics fields should be known and used by scientists and lab assistance because of their high quality and good sensitivity in experiments.