Peptidomics
About the Solution
Peptidomics involves the comprehensive study of the peptidome, which encompasses all peptides present in a biological sample. The primary analytical techniques utilized in peptidomics are liquid chromatography (LC) combined with mass spectrometry (MS). Unlike traditional methods requiring prior knowledge of biological activity, MS allows for the analysis of all endogenous small peptides, circumventing the need for bioassays or protein precursor sequence predictions.
Peptidomics shares technological similarities with proteomics but requires tailored workflows to accommodate the unique physicochemical properties of peptides, such as molecular sizes ranging from 0.5 to 10 kDa. These sizes are intermediate between typical proteins and small molecules, which historically led to peptides being overlooked in proteomic or metabolomic analyses.
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