Quantitative Proteomics

About the Infographics

Quantitative proteomics, a branch of mass spectrometry-based proteomics, is dedicated to the precise measurement and comparison of protein abundance in diverse biological samples. This comprehensive guide explores the principles, methodologies, and applications of quantitative proteomics techniques.

Key Techniques:

• iTRAQ/TMT : Isobaric labeling methods provide qualitative and quantitative insights into protein groups. Developed by AB Sciex and Thermo Fisher, these techniques offer high precision and unique ion spectra for accurate protein quantification.
• Label-Free : A technique for protein quantification that bypasses isotope labeling, analyzes peptides through liquid chromatography-mass spectrometry, and integrates precursor ion peak intensities for relative quantification.
• DIA (Data-Independent Acquisition) : A recent advancement over traditional DDA detection, DIA allows unbiased mass spectrometry analysis of all signals in complex samples. Comprehensive and unbiased, DIA captures extensive ion fragmentation information.

Are you curious about the best quantitative technique for your research? Download our PDF guide for expert insights.