Glycolysis Pathway Metabolism Assay

About the Solution

Glycolysis pathway metabolism analysis comprehensively explores cellular energy generation from glucose breakdown. This pathway involves a series of enzymatic reactions that convert glucose into pyruvate, thus producing ATP and NADH. Creative Proteomics quantifies the intermediates, maps metabolic fluxes, and assesses the enzymes in this pathway. This level of analysis provides insights on regulatory aspects of glycolysis, including substrate usage, and metabolic adjustments that further contribute to our understanding of cellular metabolism dynamics.

• Metabolite Profiling: Comprehensive analysis of glycolysis-related metabolites to quantify their concentrations and fluctuations, providing insights into pathway activity and metabolic changes.
• Metabolic Flux Analysis: Tracks the flow of carbon atoms through glycolytic intermediates using stable isotope labeling techniques, revealing metabolic flux rates and pathways.
• Isotope Tracing Studies: Employs stable isotope-labeled substrates to study the fate of glucose carbons within the glycolytic pathway, enabling the determination of metabolite turnover rates.
• Metabolic Pathway Mapping: Identification and quantification of the glycolysis pathway, elucidating the interconnected metabolic reactions.
• Metabolite Biomarker Discovery: Identification of potential biomarkers derived from glycolytic metabolites, serving as indicators of specific cellular conditions or diseases.
• Metabolite Interaction Analysis: Investigates the interactions between glycolytic intermediates and other metabolites, providing further understanding of their roles in broader metabolic networks.
• Metabolic Profiling Under Different Conditions: Comparative analysis of glycolytic metabolites under various physiological or experimental conditions, revealing regulatory mechanisms and adaptations.

You may be interested in:

• Analyzing glycolysis