N-Terminal Protein Sequencing

About the Solution

N-Terminal Protein Sequencing The N-terminus is the start of a protein or polypeptide. It influences the subcellular distribution, degradation, and the turnover rate of a protein. Therefore, the N-terminal sequence analysis of a protein is important for protein function study. In addition, in pharmaceutical proteins, there are some often observed variations of N-terminus, including incomplete processing of the N-terminal signal sequences, formation of pyroglutamate, and the lack of removal of the N-terminal methionine residue. Therefore, N-terminal sequencing is an important quality control analysis. The N-terminal sequence confirmation of drug substance is also required by the ICH Q6B.

Creative Proteomics can provide N-terminal sequencing by Edman degradation or mass spectrometry (MS), with complementary advantages to each other. We can provide Nterminal sequence analysis of peptides, therapeutic proteins, monoclonal antibodies, and protein vaccines. Download for more information.

You may be interested in:

• N-terminal Sequence Analysis of Peptides or Proteins
• Protein Sequencing by Mass Spectrometry